home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9408e.zip
/
M9480852.TXT
< prev
next >
Wrap
Text File
|
1994-09-05
|
2KB
|
40 lines
Document 0852
DOCN M9480852
TI Combination of peptide protease inhibitor and recombinant
interferon-alpha A synergistically inhibited acute and chronic HIV-1
infection in vitro.
DT 9410
AU Pagano PJ; Chong KT; Upjohn Laboratories, Kalamazoo, MI.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:485 (abstract no. T-15). Unique
Identifier : AIDSLINE ASM94/94313093
AB Effective treatment of human immunodeficiency virus (HIV) infections may
require combinations of agents that attack different molecular targets
in the viral replicative cycle. We investigated the effect of combining
U-75875, a peptide protease inhibitor containing a non-hydrolyzable
dihydroxyethylene isostere, with recombinant human interferon-alpha A
(alpha IFN). HIV-1 infected cells (H9 cells infected with HIV-1 IIIB;
human peripheral blood mononuclear cells infected with a clinical
isolate, HIV-1 JRCSF) were cultured in the presence of test compounds
and drug effects on HIV-1 p24 antigen level in supernatant were measured
by an ELISA assay. Drug interactions were then evaluated by the
MacSynergy method of Prichard and Shipman and by the median effect
principle using the combination index-isobologram technique of Chou and
Talalay. Over a range of drug concentrations (U-75875 at 0.003, 0.01,
0.03, and 0.1 microM and alpha IFN at 3, 10, 30, and 100 IU/ml), U-75875
in combination with alpha IFN synergistically inhibited the replication
of HIV-1 by both methods of synergy analysis. No significant
cytotoxicity was observed in the MTT assay at the highest concentrations
of each agent tested. We conclude that the combination of U-75875 and
alpha interferon is synergistic against both acute and chronic HIV-1
replication in vitro.
DE Cell Survival/DRUG EFFECTS Cells, Cultured Dose-Response Relationship,
Drug Drug Synergism Human HIV Core Protein p24/ANALYSIS/BIOSYNTHESIS
HIV Protease Inhibitors/*TOXICITY HIV-1/*DRUG EFFECTS/PHYSIOLOGY
Interferon Alfa, Recombinant/*TOXICITY Monocytes/CYTOLOGY/DRUG EFFECTS
Oligopeptides/*TOXICITY Virus Replication/*DRUG EFFECTS MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).